Kinetics of disappearance of kidney blood cell lineages following sublethal irradiation. (A) Light scatter profiles of whole kidney marrow cells showing the effects of 20 Gy on the relative percentages of erythrocytes (red gate), lymphocytes (blue gate), precursors (pink gate), and myeloid cells (green gate) over time. At each time point, 5 animals were killed; the mean values for each lineage are presented in the FACS plots. Representative plots were chosen from each time point based on these mean values. (B) Absolute cell counts of each leukocyte subset over time. The numbers of each population decreased to their low points by days 4 to 6. Cell counts gradually increased after this point until reaching numbers much higher than pre-irradiation levels by day 30. (C) Cytospin preparation of unirradiated (top) versus irradiated (bottom) WKM on day 7, showing that most cells within irradiated WKM display immature morphologies. Scale bar equals 10 μm.