![Figure 1. Comparison of the ability of CD20 mAbs to redistribute antigens on the cell surface. (A-B) Translocation of CD20 into the Tx100-insoluble fraction of the cell. Daudi (A) or DOHH (B) cells were incubated with FITC-labeled CD20 mAb (10 μg/mL) for 15 minutes at 37°C and washed. After chilling on ice, half of each sample was treated with 0.5% Tx100 for 15 minutes on ice. Both samples (lysed, dark gray bars; and not lysed, light gray bars) were washed and analyzed by flow cytometry (mean fluorescence intensity [MFI]) to assess bound FITC-CD20 mAbs. (C) FRET determination: Daudi cells were labeled with an equimolar mixture of Cy3 (donor)- and Cy5 (acceptor)-conjugated CD20 mAbs at 37°C with or without C8-deficient (C8-def) serum. FRET, which measures the proximity of neighboring mAb molecules, was assessed by flow cytometric analysis. These graphs are representative of at least 3 experiments, each showing similar results.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/6/10.1182_blood-2004-01-0039/6/zh80180466650001.jpeg?Expires=1735514782&Signature=iAOVWxDFIH-irCp6yjqEn7h~sIesixpzAxwXlN~tnz4sTZvXQD3mekReJI3WMBHd4eR1sbHLKKg8omzOQ5Ug9uh~J5VduzwBcD8Ng9C05uLQMEqbRZPT2K-cA2NIXF5M--10DCXmavRPF~ZNseQLV3-DQ09xYfl0leaTlzxUPh9q58GpdmQTXDFKt6BLjLz0v2~r~U7BYA93KIrm1F2aC4FZoRCGP6a-kycR80-6r-Wkt~1K0I5A3JZVDMqHRioRD7QphymgUXwXusB7eTKdUktv9eLCXzAOXmVwqMeKGQ22~dI4DNyCX2Dsv5VjdF0vCj2glTTgqtyh1QJCrefanA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Comparison of the ability of CD20 mAbs to redistribute antigens on the cell surface. (A-B) Translocation of CD20 into the Tx100-insoluble fraction of the cell. Daudi (A) or DOHH (B) cells were incubated with FITC-labeled CD20 mAb (10 μg/mL) for 15 minutes at 37°C and washed. After chilling on ice, half of each sample was treated with 0.5% Tx100 for 15 minutes on ice. Both samples (lysed, dark gray bars; and not lysed, light gray bars) were washed and analyzed by flow cytometry (mean fluorescence intensity [MFI]) to assess bound FITC-CD20 mAbs. (C) FRET determination: Daudi cells were labeled with an equimolar mixture of Cy3 (donor)- and Cy5 (acceptor)-conjugated CD20 mAbs at 37°C with or without C8-deficient (C8-def) serum. FRET, which measures the proximity of neighboring mAb molecules, was assessed by flow cytometric analysis. These graphs are representative of at least 3 experiments, each showing similar results.
