Estimation of the rates of formation and degradation of WT and Nramp2^G185R. LLC-PK₁ cells stably transfected with either WT or Nramp2^G185R-HA were labeled metabolically by a 60-minute pulse of ³⁵S-Met/Cys, followed by chase periods of the indicated length (in hours). The cells were next subjected to lysis, immunoprecipitation of Nramp2, gel electrophoresis, and autoradiography. (A) Typical radiograms of WT Nramp2 (top) and Nramp2^G185R (bottom) transfected cells. (B) Estimation of the rate and efficiency of formation of the complex-glycosylated form of WT Nramp2 (▪) and Nramp2^G185R (○). (C) Estimation of the rate of disappearance of the core-glycosylated species. Data in B and C were quantified as described in “Materials and methods” and are means plus or minus SE of 4 separate experiments.