Silencing of NmU gene expression inhibits proliferation of human myeloid leukemia cells. (A) Total RNA from K562 cells nucleofected in the absence (–) and presence (+) of siRNA was isolated 24 hours after nucleofection, reverse transcribed, and amplified in triplicate using 18S RNA (□) or NmU (▪) primers and their corresponding TaqMan probes as described in “Materials and methods.” (B) Percent cell viability of K562 cells that were mock nucleofected, or nucleofected with control siRNA (Dharmacon), or NmU siRNA was determined by trypan blue exclusion 24 hours after nucleofection. Error bars indicate standard deviation.