American Society of Hematology

Figure 6.

$Figure 6. The NmU receptor is expressed in human myeloid cells. The expression of the NMU1R was determined by Western blot analysis. Total protein (50 μg) from HL-60 cells (lane 1), peripheral blood mononuclear cells from a healthy donor (lane 2), K562 cells (lane 3), K562-MERT cells (lane 4), pheresis from a representative AML patient (lane 5), and peripheral blood from a representative ALL patient (lane 6) were fractionated by 10% SDS-PAGE, transferred to a PVDF membrane, and probed with anti-NMU1R. Along the left edge is the position of the protein standards and the arrow points to NMU1R. The solid white line between lanes 4 and 5 denotes the removal of irrelevant sample lanes from the same blot. The blot shown is representative of 3 individual determinations.$

The NmU receptor is expressed in human myeloid cells. The expression of the NMU1R was determined by Western blot analysis. Total protein (50 μg) from HL-60 cells (lane 1), peripheral blood mononuclear cells from a healthy donor (lane 2), K562 cells (lane 3), K562-MERT cells (lane 4), pheresis from a representative AML patient (lane 5), and peripheral blood from a representative ALL patient (lane 6) were fractionated by 10% SDS-PAGE, transferred to a PVDF membrane, and probed with anti-NMU1R. Along the left edge is the position of the protein standards and the arrow points to NMU1R. The solid white line between lanes 4 and 5 denotes the removal of irrelevant sample lanes from the same blot. The blot shown is representative of 3 individual determinations.

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