Mutational analysis of sequences +155 to +184. (A) The sequences of the wild-type and mutant oligonucleotides (M1-M10) corresponding to region +155 to +184 of the VWF promoter are shown. The base substitution mutations in each probe are shown, with the wild-type sequence represented as a line. (B) Gel mobility experiments were carried out with HUVEC nuclear extracts as described in Figure 2. The probes used in all samples were the wild-type oligonucleotide, and mutant oligonucleotides were used (100 × excess) as competitors. The - represents the lack of competitor. Arrow indicates the specific complex.