Analysis of the nuclear proteins bound to sequences +155 to +184. Nuclear extracts (500 μg) from HUVEC (HUV) and HEK293 (HEK) cells were used in DNA pull-down analyses as described in “Materials and methods.” Lane 1 represents the protein molecular weight marker. Lanes 2 and 3 represent flow-through proteins that were not bound to HH2 wild-type (HH2wt) probe. Lanes 4 and 5 represent eluted proteins bound to HH2 mutant (HH2mut) probe. Lanes 6 and 7 represent eluted proteins bound to HH2wt probe. Arrow in lane 6 represents the endothelial-specific protein. Positions of protein molecular weight markers are shown by arrows.