Western blot analysis of the DNA pull-down proteins from BAECs, BSMCs, and HUVECs. (A) DNA pull-down experiment and Western blot analyses were performed exactly as described for Figure 7A. Purified histone H1 was used as positive control (lane 1). Lanes 2 and 3 represent flow-through proteins that were not bound to HH2wt probe. Lanes 4 and 5 represents eluted proteins bound to HH2mut probe. Lanes 6 and 7 represent eluted proteins bound to HH2wt probe. Lane 8 represents the eluted protein from HUVECs bound to HH2wt probe. Arrow represents the endothelial-specific protein migrating approximately at 32-kDa position (shown by molecular weight marker) that is recognized by anti-histone H1 antibody. (B) Sequences of the human, bovine, and mouse VWF genes corresponding to nucleotides +1 to +250 (exon 1) are shown. Nucleotides in black boxes represent the regions of homology. The GATA and repressor NFY binding sites are shown. The ATGGCC sequence that comprises the binding site of the 32-kDa H1-like protein is shown in the open boxes.