Figure 7.
Figure 7. RT-PCR analysis for rhesus LCV latent infection gene expression in Mm256-96 tumor tissue. RT-PCR was performed on RNA extracted from the Mm256-96 tumor tissue with the use of primers specific for the mRNAs of rhesus LCV latent membrane protein 1 (LMP1), latent membrane protein 2A (LMP2A), and mRNAs spliced from the terminal exon of EBNA-LP to the coding region for EBNA-1,-2, -3A, -3B, and -3C. Control reactions consisting of all reagents except reverse transcriptase (-RT) or with water in place of RNA (W) are included. Molecular weight markers are shown in the leftmost column, with the brightest band representing 500 bp and minor bands at increments of 100 bp.

RT-PCR analysis for rhesus LCV latent infection gene expression in Mm256-96 tumor tissue. RT-PCR was performed on RNA extracted from the Mm256-96 tumor tissue with the use of primers specific for the mRNAs of rhesus LCV latent membrane protein 1 (LMP1), latent membrane protein 2A (LMP2A), and mRNAs spliced from the terminal exon of EBNA-LP to the coding region for EBNA-1,-2, -3A, -3B, and -3C. Control reactions consisting of all reagents except reverse transcriptase (-RT) or with water in place of RNA (W) are included. Molecular weight markers are shown in the leftmost column, with the brightest band representing 500 bp and minor bands at increments of 100 bp.

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