Figure 1.
![Figure 1. SHP1 gene expression and methylation in ALCL. (A) A case of ALK+ ALCL showing absence of detectable SHP1 expression in the large tumor cells, whereas the infiltrating small reactive lymphocytes are strongly SHP1-positive (immunoperoxidase, diaminobenzidine [DAB]; Olympus BX51 microscope [Melville, NY], original magnification × 400, lens 10 × with a 0.25 aperture). The image was acquired with an Optronics CCD camera and Magafire software (both from Optronics, Goleta, CA). (B) Using methylation-specific primers and a polymerase chain reaction (PCR) method, 14 of 22 cases of ALCL tumors showed detectable amplicons, in keeping with SHP1 gene methylation in these tumors.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/5/10.1182_blood-2004-03-1151/5/zh80170466190001.jpeg?Expires=1742856952&Signature=FRKdzcaPM~PfpW~kjZW4NjIcq18fYr~s9lSM8hedD8fquzMfT9iuN6u5u6asV79YqMyr-Y5JNZge7RMfgwUD-EM9yaxAVJN9UH1C0b72c9jshbGyKzkR2zEzo7WR5-YiXEpli6BK2KCCExi1a2L3U6HQZIOqEALslpv2W-YUmuqjVk-wXrmv5JKFCp7ARW8umev0Ghd5hGFmwc6-Lna7NX-CQFar3yJVKaoEfggCJeCyyoaxewGdjabWQGe-knGn1VQCcUDSZsSsJTjgY06cQq~DmYGi61Hg25XmIaRPbnRjTkVttQGeBNI34xGWkZpoXho4plQ3XJbtHfehAmHiTQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SHP1 gene expression and methylation in ALCL. (A) A case of ALK+ ALCL showing absence of detectable SHP1 expression in the large tumor cells, whereas the infiltrating small reactive lymphocytes are strongly SHP1-positive (immunoperoxidase, diaminobenzidine [DAB]; Olympus BX51 microscope [Melville, NY], original magnification × 400, lens 10 × with a 0.25 aperture). The image was acquired with an Optronics CCD camera and Magafire software (both from Optronics, Goleta, CA). (B) Using methylation-specific primers and a polymerase chain reaction (PCR) method, 14 of 22 cases of ALCL tumors showed detectable amplicons, in keeping with SHP1 gene methylation in these tumors.
