Impairment of Notch1-mediated T-cell development in vivo by DNMAML1 or Dtx1. C57BL/6 mice were lethally irradiated and reconstituted with BM cells transduced with MigR1, Mig DNMAML1, and Mig Dtx1. Analysis was performed at least 6 weeks after transplantation. (A) Flow cytometric analysis of the thymus showing GFP positivity and CD4/CD8 profile. (B) Flow cytometric analysis of the spleen showing GFP positivity in the same mice, as well as peripheral CD4⁺ and CD8⁺ cells in the GFP^- and GFP⁺ fractions. Results are representative of 3 independent experiments. The numbers in quadrants and above brackets indicate the percentage of cells.