Origin of blood cells in the 2 Gy-irradiated WBB6F₁-W/W^v mice 10 weeks after the bone marrow transplantation from B6-+/+ and B6-tg/tg mice. (A) Hemoglobin electrophoresis. Hemoglobin of WBB6F₁-W/W^v recipients after the bone marrow transplantation from B6-+/+ or B6-tg/tg mice was electrophoresed in cellulose acetate membrane. In each group, 5 mice were examined, and the results of 3 in 5 were shown. Hemoglobin of B6-tg/tg mice and that of WBB6F₁-W/W^v mice were also electrophoresed as controls. (B) Detection of transgene by genomic PCR. Genomic DNAs were purified from B and T lymphocytes, macrophages, and granulocytes, and the presence of the transgene, which was contained only in cells of B6-tg/tg mouse origin, was examined. In each group, 5 mice were examined with similar results (the result of a mouse is shown here). The presence of actin gene was examined as a control.