Treatment with E₂ does not change the phenotype of iDCs. Immature DCs, derived from monocytes cultured with GM-CSF and IL-4 for 6 days, were treated with medium only, BC (control, 20 μg/mL), or E₂ (20 μg/mL) for 24 hours. The expression of surface markers (open histogram) was detected by flow cytometry using mAbs specific for CD1a, CD80, CD86, CD40, CD83, DC-SIGN, HLA-DR, and CD14 (A). For each mAb, an isotype-specific control antibody was used (black filled histogram). Data are representative of 3 different donors. After treatment with medium alone, BC, or E₂ as described for panel A for 24 hours, the viability of the iDCs was tested by staining the cells with annexin V and PI (B). The cells were analyzed by flow cytometry. The data shown are 1 representative of at least 3 experiments.