The addition of E2 is essential for migration of mature DCs toward the lymph node-derived chemokine CCL19/MIP3β. iDCs were stimulated for 24 hours with 1μg/mL LPS in the absence or presence of 20 μg/mL BC or 20 μg/mL E2. Unstimulated and stimulated DCs were tested for their chemotactic response to CCL19/MIP3β. Migration was measured using a transwell system. Values are given as the mean number of migrated cells ± SEM and are from 1 representative experiment of 3 performed with different donors. The mean number of spontaneously migrated cells was subtracted from the total number of cells that migrated in response to the chemokine (A). Unstimulated iDCs (filled gray histogram) and iDCs stimulated with LPS alone (bold line) or in combination with E2 (thin line) were analyzed for CCR7 and CD86 surface expression by flow cytometry. The dashed line represents the staining with an isotype control antibody. Data are from 1 of 3 experiments with different donors that gave similar results (B).
