The cDCs induce secondary-like CD8⁺T-cell responses in mice that have previously received virus-stimulated pDCs. The 129sv mice primed with the DC subsets indicated in the figure (1st injection) received a second injection of GP33-loaded or unloaded DCs (2nd injection) 30 days later. One week after the second injection, the frequency of GP33-specific IFN-γ–producing cells in response to the GP33_33-41 peptide was determined by ELISPOT. The results presented are the cumulative data obtained in 4 independent experiments. Bars represent the means of 2 to 7 mice ± SDs.