The human megakaryocyte cell line, Meg-01, but not human platelets express PPARγ mRNA. Total RNA was isolated from Meg-01 cells (lane 6) and human platelets (lanes 3-5) and reverse transcribed into cDNA. The cDNA was amplified with primers specific for β-actin (539-bp product, as a control) or PPARγ (360-bp product). A 100-bp ladder was loaded in lane 1. Human adipose tissue (lane 2) and the human monocyte cell line (THP1; lane 7) were used as positive controls. Platelet samples were from a single donor (lane 3) or pooled from several donors (lanes 4-5). Reverse-transcriptase (–) controls were negative in all cases (data not shown).