The abundance of LDLR and HMG-CoAR RNAs is frequently increased in primary DNR- and ARA-C–treated AML cell samples. (A) Specific RNA levels were measured by real-time RT-PCR in aliquots of 14 primary AML samples, and significant LDLR mRNA increments and HMG-CoAR mRNA increments were measured in a majority of AML samples after DNR and ARA-C treatments, except for measured LDLR RNA increments that were not significant, in association with a smaller sample number. Error bars represent SEM, and Wilcoxon rank sum tests were used to compare means of treated and untreated AML samples along with 2-tailed tests of significance. Bold horizontal line refers to mean. (B) Real-time RT-PCR data were used to show that cholesterol increments were positively correlated with both LDLR RNA increments (r = 0.99; 95% CI, 0.67-0.99) and HMG-CoAR RNA increments (r = 0.88; 95% CI, 0.43-0.98) in ARA-C–treated AMLs, whereas neither LDLR RNA increments nor HMG-CoAR RNA increments induced by DNR were significantly correlated with cholesterol increments (LDLR r = 0.809; 95% CI, –036-0.98; HMG-CoAR r = 0.45, 95% CI –0.92-0.36).