Figure 3.
Elimination of BM Lin-Sca1+CD117+ hematopoietic progenitor and stem cells. BM cells from 15 control and 15 BM failure mice were stained with an antibody cocktail containing CD34-FITC, Sca1-PE, Lin (CD3, CD4, CD8, CD11b, CD19, Gr1, Ter119)-biotin, and CD117 (c-Kit)-APC and were analyzed using a BD-LSR flow cytometer. Data shown are expressions of Sca1 and CD117 on BM Lin- cells (top panels) and expressions of CD34 on Lin-Sca1+CD117+ BM cells (bottom panels) of control and BM failure mice.