Adhesion of neutrophils to laminin 8 and other laminin isoforms and participation of integrins. Adhesion of neutrophils to plastic surfaces coated with phosphate-buffered saline (PBS), human serum albumin (HSA), mouse laminin 1 (mLN-1), recombinant human laminin 8 (rhLN-8), and recombinant human laminin 10 (rhLN-10) in the absence or presence of 200 nM TPA was tested. mIgG (negative control), mAb IB4 (INTβ2, CD18), mAb 60.1 (INTαM, CD11b), and mAb 6S6 (INTβ1, CD29). Plastic surfaces were coated with proteins at 20 μg/mL and blocked with 2% polyvinylpyrrolidone (PVP). Adhesion-blocking mAbs were used at 20 μg/mL. Adhesion was measured after incubating the cells (100 × 103 cells/well) in the coated plates for 30 minutes at 37° C, and it is shown as optical density (OD). Mean and SD of 4 to 6 experiments are shown. P values (*P < .05; **P < .01; ***P < .001) compare mAbs with mIgG in each substrate protein. Horizontal lines compare the effect of mAbs IB4 and 60.1 on HSA and rhLN-8 in the presence of 200 nM TPA.
