Figure 2.
Figure 2. Integrity of chemokine-fused proteins. (A) MIP3αVL315 and DF2βsFv315, but not proDF2βsFv38, fusion proteins induce chemotaxis of murine CCR6-transfected HEK293 cells. Protein concentration used is shown in μg/mL. Representative data from 6 independent experiments are presented as chemotactic index (CI ± SEM of triplicate samples), defined by the fold increase in the number of migrating cells in the presence of test factors over the spontaneous cell migration. Murine MIP-3α (mMIP3α; PeproTech) was used as control. (B) vMIP2sFv38, but not vMIP2M-sFv38 or hMDCM-sFv38, binds to CCR5. Titrated amounts of proteins (0-100 μg/mL) were used to inhibit binding of 0 to 200 ng/mL human radiolabeled human MIP-1β (PeproTech) to hCCR5-transfected HEK293 cells. Unlabeled human MIP-1β (hMIP1β; PeproTech) was used as control. Data are from 2 independent experiments.

Integrity of chemokine-fused proteins. (A) MIP3αVL315 and DF2βsFv315, but not proDF2βsFv38, fusion proteins induce chemotaxis of murine CCR6-transfected HEK293 cells. Protein concentration used is shown in μg/mL. Representative data from 6 independent experiments are presented as chemotactic index (CI ± SEM of triplicate samples), defined by the fold increase in the number of migrating cells in the presence of test factors over the spontaneous cell migration. Murine MIP-3α (mMIP3α; PeproTech) was used as control. (B) vMIP2sFv38, but not vMIP2M-sFv38 or hMDCM-sFv38, binds to CCR5. Titrated amounts of proteins (0-100 μg/mL) were used to inhibit binding of 0 to 200 ng/mL human radiolabeled human MIP-1β (PeproTech) to hCCR5-transfected HEK293 cells. Unlabeled human MIP-1β (hMIP1β; PeproTech) was used as control. Data are from 2 independent experiments.

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