Binding activities of USF1 and USF2 to the TME. (A) Western blotting detection of USF1 and USF2 prepared by in vitro translation. Arrows indicate the band of either USF1 or USF2. (B) EMSA using the TME probe and USF1 and USF2 prepared by in vitro translation. The bracket indicates the specific shifted bands. NS indicates a nonspecific band. (C) EMSA was performed using the TMEmut probe. This probe includes the mutations in the E-box motifs.