Analysis of USF1 and USF2 bindings to the human PF4 promoter. (A) Comparison of the rat TME promoter with the human and mouse TME-like sequences. Homologous sequences are indicated by shaded boxes. Dashes indicate deletion sequences. The bracket below the sequence indicates the conserved E-box motif. EMSA was performed using hTME-like (B), hTMEmut (C), hE-box (D), or hE-boxmut (E) probes. Arrows indicate the specific shifted bands. NS indicates a nonspecific band. The hTMEmut or hE-boxmut probes were prepared by mutating the E-box motif of the hTME or hE-box probe, respectively.