PSGL-1 cross-linking up-regulates αMβ2. (A) Neutrophils were incubated with 10 μg/mL sP-selectin (sP-sel), P-selectin Ig chimera (P-sel Ig), and P-selectin Ig chimera cross-linked by F(ab′)2 fragment of anti–human IgG (Fc specific; anti-hIgG), respectively. The differences between sP-sel and control were statistically insignificant (P > .05), whereas the differences between P-sel Ig or P-sel Ig plus anti-hIgG and control were statistically significant (P < .01). (B) Neutrophils were treated with 10 μg/mL Fab or F(ab′)2 fragment of PL1. They were then transferred into the wells coated with fibrinogen. Other steps of the cell adhesion assay were same as for Figure 1. All results are expressed as the mean ± SD values of the adherent cells determined in triplicate measurements of 3 separate experiments. The differences between Fab and control were statistically insignificant (P > .05), whereas the differences between F(ab′)2 and control were statistically significant (P < .01).
