c-IAP1 redistribution involves a leptomycin B-sensitive mechanism. (A) Fluorescence microscopy analysis of c-IAP1 expression (Pharmingen mAb; green) in THP1 cells treated with 20 nM TPA for 24 hours in the presence or absence of 100 nM leptomycin B (LMB). Hoechst 33342 was used to stain the nuclei (blue). (B) HeLa cells were transiently transfected with a GFP-c-IAP1 construct before staining the nuclei with Hoechst 33342 and fluorescence microscopy analysis. When indicated, LMB (200 nM) was added 3 hours before analysis. Magnification is × 500 for panels A and B.