Figure 1.
Figure 1. Induction of EPOR by hypoxia in TrHBMECs. (A) EPOR expression was determined by quantitative real-time RT-PCR in TrHBMECs at 21% and 2% O2 without (□) and with (▪) EPO (5 U/mL). Compared with the EPOR level in 21% O2, we observed a 5-fold maximal induction of the EPOR after 48 hours of treatment with EPO at 2% oxygen (***P < .001). Results were normalized to β-actin expression. (B) Western blotting confirmed EPO induction of EPOR protein for cells cultured at 2% O2 for 48 hours. Error bars represent SD.

Induction of EPOR by hypoxia in TrHBMECs. (A) EPOR expression was determined by quantitative real-time RT-PCR in TrHBMECs at 21% and 2% O2 without (□) and with (▪) EPO (5 U/mL). Compared with the EPOR level in 21% O2, we observed a 5-fold maximal induction of the EPOR after 48 hours of treatment with EPO at 2% oxygen (***P < .001). Results were normalized to β-actin expression. (B) Western blotting confirmed EPO induction of EPOR protein for cells cultured at 2% O2 for 48 hours. Error bars represent SD.

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