Figure 2.
Induction of eNOS by EPO in TrHBMECs. (A) eNOS expression was determined by quantitative real-time RT-PCR without (□) or with (▪) EPO (5 U/mL) or VEGF (5 pg/mL; ▨) at 21% O2 and 2% O2 for 48 hours. eNOS expression was induced following treatment with EPO at 2% O2 compared with expression at 21% (***P < .001) but was not induced following treatment with VEGF. Results were normalized to β-actin expression. (B) Western blotting confirmed greater induction of eNOS protein in cells cultured for 24 hours at 2% O2 in the presence of EPO. Error bars represent SD.
