DC morphology. (A) Immature monocyte-derived DC. (B) Monocyte-derived DC matured with IL-1β, IL-6, tumor necrosis factor α (TNFα), and prostaglandin E₂ (PGE₂). Dif-Quick–stained cytocentrifuge preparations are shown. Viewed at 1000× magnification using an Olympus BX51 microscope with an Olympus U Plan Fluorite 100×/1.30 NA oil immersion objective. Images were captured using an Optronics MicroFire digital camera and MicroFire image acquisition software and processed with Adobe Photoshop.