Figure 2.
Mitochondrial permeability transition and Bid cleavage during neutrophil apoptosis. (A) Freshly isolated neutrophils and neutrophils cultured for 6, 9, or 20 hours were loaded with DiOC6 or JC-1 and analyzed for uptake and retention of the dyes by FACS analysis. A representative of 4 experiments is shown. (B) Lysates were prepared from freshly isolated neutrophils and neutrophils cultured for 0, 3, 6, 9, 12, and 18 hours and analyzed by Western blotting for the presence of full-length 22-kDa Bid and the cleaved 15-kDa form. A representative of 3 experiments is shown.
