Expression of endothelial and hematopoietic cell markers in cultured cord blood CD34⁺ cells. (A-F) Immunofluorescence of cord blood CD34⁺ cells at 7 days (A-B) and 21 days of culture (E-F), stained for VE-cadherin (A,C) and Tie-2 (B,D). (E) Control goat IgG. (F) Control rabbit IgG. Bar is 28 μm. (G) Flow cytometric analysis of cord blood–derived endothelial cells at 21 days of culture stained for VE-cadherin, CD34, CD31, CD45, CD14, and CD133. The vertical line in each histogram marks the upper limit of IgG isotype controls.