Expression of TRAIL-Rs in adherent human PBMCs and in RAW264.7. PBMCs were let to adhere for 3 to 5 days before performing Western blot and phenotypic analysis. At this time point, adherent PBMCs were either stained for monocytic/macrophagic markers CD14, CD36, and CD64 (A) or analyzed for TRAIL-R expression (B, C). (A) Dot plot shows the forward and side scatter (FSC/SSC) profile and the gate, on viable cells, considered for the phenotypic analysis. In (B) TRAIL-R expression was evaluated in adherent PBMCs and in RAW264.7 by Western blot analysis. A representative of 3 separate experiments is shown. (C) Surface TRAIL-R expression was evaluated in adherent PBMCs by flow cytometry. A representative of 7 separate experiments is shown. In panels A and C, shadowed histograms represent cells stained with MoAbs specific for the indicated surface antigens (CD14, CD36, CD64, and TRAIL-Rs) whereas unshadowed histograms represent the background fluorescence obtained from the staining of the same cultures with isotype-matched control MoAbs.