Production of cytokines by splenocytes from AD- or chow-fed WT mice treated with α-GalCer. Splenocytes were obtained from either AD- or chow-fed WT mice 2 or 12 hours after intravenous injection with 0.1 μg/g BW α-GalCer. Cells were cultured for 1.5 hours without additional stimulation. Culture supernatants were harvested, and IFN-γ, IL-4, and IL-10 levels were quantitated. Values are mean ± SE. Statistical analyses were performed using the Mann-Whitney U test. ^*P < .05; ^**P < .01.