Protein oxidation in marrow progenitors and peripheral red cells. Protein carbonyl content in Sod2^+/+ and Sod2^–/– samples was compared by Oxyblot. Lane 1 (Sod2^+/+) and lane 2 (Sod2^–/–) compare whole-cell lysate protein from 5 × 10⁶ Ter119⁺ marrow progenitor cells isolated by FACS. Lane 3 (Sod2^+/+), lane 4 (Sod2^–/–), and lane 5 (Sod2^–/– Euk-189–treated) contain 20 μg red cell cytosolic protein, and lane 6 (Sod2^+/+), lane 7 (Sod2^–/–), and lane 8 (Sod2^–/– Euk-189–treated) contain 5 μg membrane protein from the corresponding red cell samples.