The T-cell subsets that were compared with GC-Th cells in the microarray study. All T-cell subsets used in the study were highly purified cells from peripheral blood (naive, CXCR5+CCR7+ memory, and CCR7-effector memory cells) and tonsils (CD57+ GC-Th cells). Cells were isolated by a combination of magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting (FACS). The surface markers used for cell isolation, proposed function, and typical polarization status of T-cell subsets are shown.