Clone HLA restriction and antigen specificity. (A) Adenovirus-specific CD8⁺ T-cell clones from donor AL are A*1 and A*24 restricted. Adenovirus-specific CD8 T-cell clones c11 and c16 were incubated with autologous and partially HLA class I–matched LCL targets infected with Ad5f35GFP; only shared alleles are indicated. Results are expressed as SFCs per 100 T cells. (B) In vitro–reactivated adenovirus-specific T-cell clones recognize a capsid protein, hexon. (i) A1-restricted clone 11 tested at E/T ratios of 5:1 and 2.5:1. (ii) A*24-restricted clone 16 tested at E/T ratios of 30:1, 20:1, and 10:1. Killing was assessed against autologous fibroblast targets alone or fibroblasts transduced with the MSCV–Hexon–internal ribosome entry site [IRES]–GFP viral supernatant (retro-Hexon) or with MSCV-Penton-IRES-GFP viral supernatant (retro-Penton). Error bars reflect standard deviation of triplicates.