Figure 3.
HLA-A*1–restricted adenovirus-specific T-cell clones are both sub-group and serotype cross-reactive. (A) Cytolytic activity of adenovirus-specific, A*1-restricted clone 11 (E/T 20:1) against autologous and allogeneic fibroblast targets either alone or transduced with the indicated Ad serotypes. (B) Epitope alignment of sequences from the indicated Ad serotypes. (C) Hexon-specific, A*1-restricted T-cell response (clone 11 from donor AL) by ELISPOT assay. Stimulators are autologous LCLs pulsed with 1 μg/mL of the synthesized peptides, designated by the first 3 amino acids of the peptide sequence. Results are expressed as SFCs per 1 × 104 T cells.
