HLA-A*24–restricted adenovirus-specific T-cell clone 16 is subgroup C and D specific. (A) Cytolytic activity of adenovirus-specific, A*24-restricted clone 16 (E/T 20:1), incubated with autologous and allogeneic fibroblast targets, either alone or transduced with the indicated Ad serotypes. (B) Alignment of peptide sequences from the indicated Ad serotypes. (C) Hexon-specific, A*24-restricted T-cell response (clone 16 from donor 1) by ELISPOT assay. Autologous LCL targets were pulsed with 1 μg/mL of the indicated peptides and then used as stimulators in an ELISPOT assay, with clone 16 T cells serving as responder cells. Results are expressed as SFCs per 5 × 10³ T cells.