Cross-reactivity and frequency of HLA-B*7–restricted adenovirus-specific T-cell responses. (A) Cytolytic activity of adenovirus-specific, MPN-specific B*7-restricted clone 32 (E/T 20:1) against autologous and allogeneic fibroblast targets either alone or transduced with the indicated Ad serotypes. (B) Cytolytic activity of adenovirus-specific, KPY-specific B*7-restricted clone 12 (E/T 20:1) against autologous and allogeneic fibroblast targets either alone or transduced with the indicated Ad serotypes. (C) PBMCs (day 0) and CTL lines (day 9) from B*7-positive donors (6 and 12) were screened for the percentage of KPY-specific tetramer-positive T cells.