Figure 1.
Mouse hematopoietic progenitor cells express α4β7 integrin complex. (A) Flow cytometry gating of bone marrow–derived Lineageneg Sca-1pos c-kitpos (LSK) cells. (B) Selectin ligands, CXCR4, and α4 integrin expression on bone marrow LSK cells and on FDCP-mix mouse progenitor cell line. Filled histograms represent negative controls from isotype-matched staining or selectin chimeric protein staining in the presence of 5 mM EDTA (ethylenediaminetetraacetic acid). Geometric mean values obtained after subtraction of isotype-matched control values from 3 independent experiments were as follows: LSK cells, 576 ± 33 for α4 integrins and 31 ± 7 for α4β7 integrin; FDCP-mix cells, 656 ± 37 for α4 integrins and 18 ± 7 for α4β7.
