Figure 2.
The α4β7 integrin on FDCP-mix is functional. Fluorescently labeled FDCP-mix cells were preincubated with 10 μg/mL of control rat IgG, antibodies against the α4β7 integrin (clone DATK32), or α4 integrins (clone PS/2). Adhesion to MAdCAM-1–Fc was then allowed to proceed in the absence or presence of 100 ng/mL of CXCL12 for 30 minutes at 37°C. Bars represent mean percentage of adhesion ± SEM from 3 independent experiments. *P < .05; **P < .01 compared with the respective rat IgG controls or between indicated 2 groups. Irrelevant binding antibody did not influence adhesion, as anti–L-selectin (Mel-14) antibody-treated FDCP-mix cells were 105.7% ± 5.5% that of rat IgG-treated FDCP-mix cells (n = 3 experiments).
