Correlation between Foxp3 gene expression and de novo T-cell development. (A) T-cell receptor gene rearrangement excisional circle (TREC) levels from PBMCs of patients without clinical manifestations of alloGVHD (n = 8), patients with alloGVHD (n = 28), patients evaluated at the onset of autoGVHD (n = 16), patients without autoGVHD (n = 23), and non-CsA-treated autoBMT patients (n = 6). Error bars indicate SEM. (B) Correlation between TRECs and Foxp3 expression in PBMCs of healthy controls (n = 10) and patients undergoing alloBMT (assessed < 500 days after transplantation) or undergoing autoBMT with autoGVHD induction. Relative expression of Foxp3 or TRECs was normalized against GAPDH or RNaseP, respectively. Regression lines correspond to coefficient correlations (top: healthy individuals, middle: autoBMT patients, and bottom: alloBMT patients).