Figure 5.
Suppression of the CD8+ T-cell response. CD8+ T cells (1 × 105) were stimulated with IL-2 (100 U/mL) in the presence of irradiated auto 5 × 103 DCs. CD4+Foxp3+ T cells generated from CLIP-pulsed DCs were added to the assay as suppressor cells at a ratio of 1:1. The cells were cultured 72 hours and pulsed with [3H] thymidine for the 8 hours of culture. Proliferation data are expressed as the mean ± SD from triplicate wells and represent 3 experiments.