Mutant PILSAP inhibits angiogenesis in vivo. A mock or E343A expression plasmid was incorporated into the HVJ-envelope vector and mixed with Matrigel. Matrigel was subcutaneously injected into the abdomen of C57BL/6 mice. Sections were observed using an Olympus BH2 microscope and a high-resolution CCD color camera. Objective lens: SPlan 20×; camera: CCD Digital3 CCD camera KS630; software: Image-Processing (FLOVEL, Tokyo, Japan). The hemoglobin content of the Matrigel was determined (n = 4; *P < .01; **P < .005). Error bars indicate SDs.