Analysis of apoptosis in CKR516-STAT1β–transfected cells treated or not treated with fludarabine. (A) Analysis of the sub-G1 peak. EGFP-positive and -negative cell populations purified by FACS after doxycycline treatment were further treated with 15 μM fludarabine for 16 hours or were left untreated. DNA content was assessed by flow cytometry using the 7AAD dye after ethanol fixation, allowing quantification of the sub-G1 peak. (B) Analysis of PARP cleavage. Detection of the PARP fragment cleaved by caspase 3 was performed by Western blotting of total protein extracts from cells treated (+) or not treated (–) with 0.6 μg/mL doxycycline for 24 hours and treated (+) or not treated (–) with fludarabine for 24 and 72 hours. Results are representative of at least 2 independent experiments.
