FACS analysis of BCR/ABL retroviral transduction efficiency of wild-type and P-selectin^-/-/ICAM1^-/- bone marrow cells. (A) Wild-type and P-selectin^-/-/ICAM1^-/- mice have similar percentages (mean values ± SD) of myeloid (Gr-1- and Mac-1-positive) and lymphoid (B220- and CD19-positive) progenitor cells and hematopoietic stem cells (c-kit- and Sca-1-positive) in bone marrow. One-way ANOVA revealed no statistically significant differences between the 2 groups (P > .05 for all markers). (B) Bone marrow cells from wild-type and P-selectin^-/-/ICAM1^-/- mice were equally susceptible to transduction by BCR/ABL retrovirus. Bone marrow cells were transduced with BCR/ABL retrovirus, cultured with IL-3, IL-6, and stem cell factor for 2 days, and analyzed by FACS for total GFP-positive cells.