Figure 1.
Human MDS marrow cells in mouse blood, bone marrow, and spleen. The slide was mounted on Vectashield from Vector Laboratories (Burlingame, CA). A Nikon E600 microscope (Nikon, Melville, NY) was used, equipped with epifluorescence with appropriate double and triple band pass filters for visualization of FITC, Texas Red, Spectrum Orange, Spectrum Green, or DAPI (Nikon Plan Fluor 100×/1.30 oil immersion oil from Criterion Sciences, Riverdale, NJ). Cells were photographed using a Nikon FDX-35 camera with Kodak Professional Ektachrome P 1600 film. The film was developed by Prolab. The slide was scanned on a Nikon Super Coolscan 4000 with no corrections at 2000 dpi and adjusted for levels, color, and size in Photoshop (Adobe, San Jose, CA).