Figure 3.
Figure 3. Time course of APS IgG-induced monocyte TF mRNA expression and enzymatic activity. (A) TF mRNA expression in monocytes was measured by real-time RT-PCR (TaqMan). Normal monocytes were incubated with IgG (250 μg/mL) from a patient with APS or a healthy individual, in the presence of β2GPI (100 μg/mL). Stimulation with LPS (500 ng/mL) was performed as a positive control. Fold change is relative to the expression level at time zero. Data shown are the mean of triplicate determinations. (B) TF activity on monocytes was measured as factor VIIa-dependent activation of factor X. Normal monocytes were incubated with IgG (250 μg/mL) from a patient with APS or a healthy individual, in the presence of β2GPI (100 μg/mL), and TF activity was measured at 0, 2, 4, 6, and 24 hours. Data shown are the mean ± SEM of duplicate determinations.

Time course of APS IgG-induced monocyte TF mRNA expression and enzymatic activity. (A) TF mRNA expression in monocytes was measured by real-time RT-PCR (TaqMan). Normal monocytes were incubated with IgG (250 μg/mL) from a patient with APS or a healthy individual, in the presence of β2GPI (100 μg/mL). Stimulation with LPS (500 ng/mL) was performed as a positive control. Fold change is relative to the expression level at time zero. Data shown are the mean of triplicate determinations. (B) TF activity on monocytes was measured as factor VIIa-dependent activation of factor X. Normal monocytes were incubated with IgG (250 μg/mL) from a patient with APS or a healthy individual, in the presence of β2GPI (100 μg/mL), and TF activity was measured at 0, 2, 4, 6, and 24 hours. Data shown are the mean ± SEM of duplicate determinations.

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