Proliferation in response to aAg stimulation of naive and memory T-cell subsets. Donor cells (from donor 1, a representative sample) were isolated and expanded in culture for 7 days to obtain sufficient cell numbers. T-cell subsets were then cocultured against 3 separate irradiated HLA-mismatched LCLs for 6 days and proliferation measured by thymidine incorporation. Error bars represent the SEM for donor 3 against 3 separate LCLs. Statistical significance is shown by brackets comparing CD62L^– against T_N (P = .01) and T_CM (P = .04).