Hierarchic clustering supervised by PAM analysis of FLT3-WT versus FLT3-ITD and FLT3-ALM, and FLT3-ITD versus FLT3-ALM samples. (A) The hierarchic clustering defined by PAM analysis of FLT3-WT versus FLT3-ITD samples; overall misclassification error is 11%. The difference in EFS between these 2 branches is 48% versus 24% (P = 0.1). (B) The differential gene expression between FLT3-WT and FLT3-ALM samples had a higher overall classification error rate, and was unable to differentiate FLT3-ALM samples from all FLT3-WT samples. (C) We show hierarchic clustering of FLT3-ITD versus FLT3-ALM samples. Overall misclassification rate is 19% and differences in gene expression may be related to their different clinical behavior, and to differences in mean age and mean WBC at presentation.