Hierarchic clustering supervised by PAM analysis of FLT3-MUs versus FLT3-WT in 72 diagnostic samples. In this group of patients, FLT3-MU is not a universal prognostic factor of poor outcome because of the IR/R/CCR outcomes in WT and MU FLT3 signature groups. (A) Hierarchic clustering defined by PAM analysis did not include any known genes related to the FLT3 receptor signal transduction pathway. There are 6 samples with the FLT3-WT receptor that have a gene expression profile similar to that of FLT3-MU signature, independent of the FLT3 receptor genotype. One FLT3-ITD also has a gene expression profile similar to FLT3-WT signature, independent of the FLT3 receptor genotype. (B) Pairwise comparison of FLT3-MU versus FLT3-WT shows a trend for different EFS, but it does not achieve statistical significance defined by mutation status or gene expression signatures.