Function of E1-CTLs following transduction with adenovectors. (A) There were 2 CTL and LCL cell lines transduced for 6 hours with Ad5/F35-GFP using 1000 vp per cell. At 24 to 48 hours after infection, the cells were stained with a PE-CD3 or PE-CD20 antibody and analyzed by FACS. The results are expressed as the percentage of cells expressing CD3 and GFP. Ad5F35 transduced 51% to 58.5% of the CTLs and 44% to 48.5% of the LCLs. (B) The killing activity of CTLs is not changed by double transduction. The cytotoxic activity of doubly transduced CTLs (C-RE-AG, □) was compared with nontransduced CTLs (▪). The graph shows the percentage of specific lysis at an effector to target ratio of 20:1 against autologous LCLs, allogeneic LCLs, and a complete HLA class I mismatch control target line (H-2B). Measurement at other ratios (40:1 and 10:1) was also unchanged. Results are shown as mean ± SD.